Our AVI-PDX ™and AVI-cell-DX™ models
OncoFactory operates a unique technological platform exploiting an innovative patient-derived xenograft (PDX) animal model, the avian embryo. Miniature replicas of tumors from patient samples (AVI-PDX™) or from cell lines (AVI-cell-DX™) are created in a series of embryos, which can receive therapeutic treatments. Our technology allows modeling the heterogeneity of patient tumors, statistical analysis of treatment efficacies, and a range of molecular analyses for biomarker and therapeutic target discovery. The AVI-PDX model of Oncofactory holds the unique advantage of allowing the creation of tumors and the delivery of therapeutic compounds in only a few days. Moreover, the tumorigenesis is targeted onto tissues which are homologous to those in which primary tumors emerge in the patient or in known sites of metastasis. Tumor replicas are imaged in 3D in the entire organism by using high resolution confocal microscopy. A range of molecular analyses can also be conducted on tumor replicas, host tissues and fluids.
This technology is protected by 2 patents :
1.EP 3 167 048 B1
- Tumorigenesis is targeted into selected territories corresponding to patient primary tumor sites or to known metastatic sites.
- Cancer cells exploit tissue specific signals of the embryo to proliferate and to establish metastases.
What can we do with the OncoFactory technology?
- create a vast range of PDX models from patient samples; including for cancers that failed to develop in classical patient-derived xenograft mice
- model the heterogeneity of patient tumors for validation of targeted therapies
- identify responsive patients and search for markers distinguishing them among the population of patients.
How and why does our model work?
Classically, patient-derived xenografts (PDX) are achieved under the skin of adult immune-deprived mice and take weeks to months to give rise to tumors. The dermis environment strongly differs from that of tissues in which tumors form in the patients and is poor in signals important for tumors to develop. In contrast, the embryonal tissues are rich in signals, different from one tissue to the other one, which ensure their building. The same signals will mediate tissue renewal at adulthood. Thus, tumoral cells can recognize these signals in the embryo and exploit them to proliferate and disseminate. By selecting the tissue in which tumors will form, we confront tumoral cells to an environment which evokes that of their site of origin in the patient. This makes it our model powerful to generate tumors in an optimal range of time. Delloye-Bourgeois et al., Cancer Cell, 2017
-Advantages on existing PDX models-
Mouse PDX model
Tumoral masses in 48h, Metastasis in 2 to 7 days
One patient/ series of standard replicas
REPRODUCIBLE ORTHOTOPIC TUMORS
Tumorigenesis targeted in tissues homologous to those of the patients 100% tumor intake from patient samples
EASY ETHIC PROCEDURE, HIGH FLEXIBITY
2013 86/609/EEC rule, no need for protocol approval
EASY SAMPLE ENGINEERING
Reduced biological sample Biobanking
Tumors in several weeks to months
One patient/ a few mice
Versatile heterotopic tumors
Highly variable tumor intake Irrelevant tissue environment (subcutaneous sote)
Complex ethic procedure, Low flexibility
Protocol approval by Ethic committee
Complex sample engineering
Substantial amount of fresh patient sample